Abstract：

Current protocols to differentiate human induced pluripotent stem cells (h-iPSCs) into endothelial cells (h-iECs) lack reliability. Here we describe a method for rapid, consistent and highly efficient generation of h-iECs from h-iPSCs. The protocol entails delivery of modified mRNA encoding the transcription factor ETV2 at the intermediate mesodermal stage of differentiation. This approach reproducibly differentiated thirteen diverse h-iPSC lines into h-iECs with exceedingly high efficiency. In contrast, differentiation with the standard protocol, which relies on endogenous ETV2, was inefficient and notably inconsistent. Our method generated h-iECs that were functionally competent in many respects, including ability to form perfused vascular networks in vivo. Importantly, timely activation of ETV2 was critical and bypassing the mesodermal stage produced putative h-iECs with reduced expansion potential and that lacked ability to form functional vessels. Our protocol could have broad application in regenerative medicine and reliably provide an unlimited number of autologous h-iECs for vascular therapies.

报告人简介： 

王凯，2010年本科毕业于东南大学生物医学工程系，2016年获得6163am银河线路生物医学工程系博士学位，主要研究免疫隔离装置的生物相容性改性研究和组织工程支架递送胰岛细胞治疗糖尿病的研究。现在哈佛医学院心脏外科系接受博士后训练。研究集中在利用化学改性信使RNA高效诱导人胚胎干细胞分化成血管内皮细胞的方法，为临床提供大量可供移植的自体内皮细胞。在包括Advanced Materials，Biomaterials等国际顶级期刊发表SCI论文10篇；受邀在包括MRS和NAVBO等国际研讨会上作报告，荣获最佳口头报告奖；作为科研骨干，参与美国NIH自然科学基金。协助导师获得JDRF（美国青少年糖尿病基金会）共计75万美金的研究基金；同时也是若干国际知名期刊的审稿人。

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